Actinotalea suaedae sp. nov., Isolated from the Halophyte Suaeda physophora in Xinjiang, Northwest China
2015-02-05
The genus Actinotalea, belonging to the family Cellulomonadaceae, currently contains only two species with validly published names, Actinotalea fermentans DSM 3133T and A. ferrariae CF5-4T.
LI Zhaoshuai et al. collected a halophyte plant Suaeda physophora from dry inflorescences in natural population growing in desert saline soil near the Fukang Field Research Station of the Chinese Academy of Sciences, and isolated a Gram-stain-positive, aerobic, nonmotile, coryneform bacterium, designated strain EGI 60002T, from the halophyte S. physophora.
Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to A. ferrariae CF5-4T (95.8% gene sequence similarity). The peptidoglycan type of strain EGI 60002T was A4β, containing L-Orn-D-Ser-D-Asp. The cell-wall sugars were mannose, ribose, rhamnose and glucose. The major fatty acids (>5%) of strain EGI 60002T were iso-C14:0, iso-C15:0, anteiso-C15:1 A and anteiso-C15:0. The predominant respiratory quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol (DPG), one unidentified phosphoglycolipid (PGL) and one unidentified phospholipid (PL1). The genomic DNA G+C content was 72.3 mol%. On the basis of morphological, physiological, chemotaxonomic data, and phylogenetic analysis, strain EGI 60002T should be classified as a novel species within the genus Actinotalea, for which the name A. suaedae sp. nov. is proposed. The type strain is EGI 60002T (=JCM 19624T = KACC 17839T = KCTC 29256T).
The result was published in Antonie van Leeuwenhoek in January 2015.